Application

Research CategorySignaling

Research Sub CategorySignaling Neuroscience

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Nucleobindin-2 in human normal colon, human breast cancer tumor cells, rat small intestine, and mouse hippocampus tissue.

Detect Nucleobindin-2 using this Anti-Nucleobindin-2 Antibody, clone 11B3.1 validated for use in Western Blotting and Immunohistochemistry (Paraffin).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nucleobindin-2 (UniProt P80303; also known as DNA-binding protein NEFA, Epididymis secretory protein Li 109, Gastric cancer antigen Zg4, Nucleobinding 2, Prepronefastin, Prepronesfatin) is encoded by the NUCB2 gene (also known as HEL-S-109, NEFA) (Gene ID 4925) in human. Nucleobindin-2 is initially translated as a 420 aa precursor protein. Cleavage of signal peptide (aa1-24) yields the 396 aa mature nucleobindin-2 with very high sequence homology among human, mouse, and rat species (>85%). Post-translational processing by prohormone convertases (PC 1/3 and PC 2) cleaves nucleobindin-2 into nesfatin-1, nesfatin-2, and nesfatin-3 (aa1–82, aa85–163, and aa166–396, respectively, numbering based on mature sequence). Nesfatin-1 is a well recognized circulating anorexigenic peptide, whose production increases post-prandially. Nucleobinin-2 contains two calcium-binding EF-hand domains separated by an acidic amino acid-rich region, and a leucine zipper. NUCB2 is expressed in key hypothalamic nuclei and plays a role in energy homeostasis. It is also expressed in various human peripheral tissues, including the stomach, pancreas, reproductive organs, and adipose tissue, suggesting its roles in various metabolic functions. NUCB2 expression level is reported to be elevated in clear cell renal cell carcinoma (ccRCC), breast cancer, and prostate cancer (PCa) tissues.

Immunogen

GST-tagged recombinant protein corresponding to human Nucleobindin-2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nucleobindin-2 in 10 µg of Jurkat cell lysate.

Specificity

Detects nucleobindin-2, but not nesfatin-1. Reactivity toward nesfatin-2 or nesfatin-3 has not been determined.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 50 kDa observed